Cloning and Characterization of the DNA Region Responsible for 1 Megacin A - 216 Production in Bacillus megaterium 216
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چکیده
Cloning and Characterization of the DNA Region Responsible for 1 Megacin A-216 Production in Bacillus megaterium 216 2 3 Antal Kiss, Gabriella Balikó, Attila Csorba, Tungalag Chuluunbaatar, 4 Katalin F. Medzihradszky, and Lajos Alföldi 5 Institute of Biochemistry, Proteomics Research Group, Institute of Genetics, Biological 6 Research Center of the Hungarian Academy of Sciences, Szeged, Hungary; Department 7 of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 8 94143-2240, U.S.A. 9 10 11 12 Running title: Bacillus megaterium megacin A-216 region 13 14 15 16 *Corresponding author. Mailing address: Institute of Biochemistry, Biological 17 Research Center of the Hungarian Academy of Sciences, 6726 Szeged, Temesvári krt. 18 62., Hungary. Phone: +36 62 599 630, Fax: +36 62 433 506, E-mail: [email protected] 19 A. C. present address: Inst. of Medical Chemistry, Szeged University, 6720 20 Szeged, Dóm tér 8., Hungary 21 AC CE PT ED Copyright © 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. J. Bacteriol. doi:10.1128/JB.00557-08 JB Accepts, published online ahead of print on 8 August 2008
منابع مشابه
Cloning and characterization of the DNA region responsible for Megacin A-216 production in Bacillus megaterium 216.
Upon induction, Bacillus megaterium 216 produces the bacteriocin megacin A-216, which leads to lysis of the producer cell and kills B. megaterium and a few other bacterial species. The DNA region responsible for megacinogeny was cloned in B. megaterium. The nucleotide sequence of a 5,494-bp-long subfragment was determined, and the function of the genes on this fragment was studied by generating...
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A host-vector system was developed for molecular cloning in Bacillus megaterium and used to clone the structural and immunity genes for megacins A-216 and A-19213. Recombinant clones that expressed immunity only or both immunity to and production of each megacin were obtained. Restriction mapping of native megacinogenic plasmids and recombinant clones was used to construct physical and genetic ...
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A bacteriocin produced by Bacillus megaterium ATCC 19213 was identified, purified, and compared with megacin A from B. megaterium 216. The ATCC 19213 bacteriocin was inducible with mitomycin C and showed phospholipase A activity. Both megacin A-216 and megacin A-19213 contained two dissimilar polypeptide subunits. Megacin A-216 contains a 30,000-dalton alpha subunit and a 15,000-dalton beta sub...
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